Effect of progesterone on esterification of free cholesterol, on content of free cholesterol and its esters was studied in rat macrophages, cultivated in presence of acetyl-derivatives of low density lipoproteins (Ac-LDL), as well as on mobilization of cholesterol from macrophages. Progesterone at a dose of 0.5-10 micrograms per I ml of cultivating medium caused dose-dependent inhibition of I-14C-oleate incorporation into cholesterol esters of macrophages cultivated with Ac-LDL (50 micrograms of protein/ml). In the medium free of LDL progesterone caused an increase of cholesterol in macrophages by 45%, while addition of 100 micrograms/ml LDL led to a decrease of free cholesterol by 20% of the initial level. If macrophages pre-enriched with 4-C14-cholesterol were cultivated in LDL containing medium, progesterone increased the 4-14C-cholesterol liberation into medium and decreased the cholesterol content in macrophages. Under conditions of macrophages incubation in the LDL-free medium, progesterone increased the free cholesterol content in these cells but did not affect the 4-14C-cholesterol liberation into medium. The data obtained suggest that the progesterone-induced inhibition of free cholesterol esterification may increase its mobilization from macrophages only if the LDL acceptor function is unaltered.
Dolgov A.V. et al. The effect of progesterone on metabolism of cholesterol esters in macrophages cultured with acetylated low density lipoproteins // Voprosy meditsinskoi khimii. - 1990. - V. 36. -N 4. - P. 38-41.
Dolgov A.V. et al., "The effect of progesterone on metabolism of cholesterol esters in macrophages cultured with acetylated low density lipoproteins." Voprosy meditsinskoi khimii 36.4 (1990): 38-41.
Dolgov, A. V., Dushkin, M. I., Mandrikova, E. V. (1990). The effect of progesterone on metabolism of cholesterol esters in macrophages cultured with acetylated low density lipoproteins. Voprosy meditsinskoi khimii, 36(4), 38-41.