ATP-generating creatine kinase: a new component discovered newly in viperidae venom

   
Kouznetsov D.A.1, Orlova M.A.2 , Berdieva A.G.3, Khasigov P.Z.3

1. Semenov Institute of Chemical Physics, RAS
2. Lomonosov Moscow State University, Russia
3. Sechenov Moscow Medical Academy Russia
Section: Short Communication
PubMed Id: 14565086
Year: 2003  Volume: 49  Issue: 2  Pages: 213-217
This is a first report on the presence of creatine kinase, ATP- generating enzyme, in the venom of Vipera xanthia representing the Viperidae family of poisoning snakes. Kinetic and catalytic properties of 40 kD active monomer has been described with a following discussion on the issue of potential of the data listed for further research in either toxic coagulopathy molecular mode or enzyme application dealing with "thrombin - thrombin receptor" interaction studies. An enzyme purification procedure includes hydrophobic (phenyl-Sepharose), anion exchange (MonoQ) and affinity (ADP-Sepharose) chromatographic steps.
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Kouznetsov, D. A., Orlova, M. A., Berdieva, A. G., Khasigov, P. Z. (2003). ATP-generating creatine kinase: a new component discovered newly in viperidae venom. Biomeditsinskaya khimiya, 49(2), 213-217.
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