Free radical processes intensity and regulation of cytoplasmic NADP-dependent malate dehydrogenase in rat cardiomyocytes at norm and under ischemia

   
Safonova O.A.1 , Popova T.N.1, Matasova L.V.1, Artyukhov  V.G.1

1. Voronezh State University
Section: Experimental/Clinical Study
PubMed Id: 16104394
Year: 2005  Volume: 51  Issue: 3  Pages: 311-320
Experimental ischemia of rat myocardium was accompanied by increase of light sum (S) and maximal intensity (Imax) of chemiluminescence, amount of a malonic dialdehyde and conjugated dienes in cytoplasmic fraction. The activity of NADP-dependent malate dehydrogenase (EC 1.1.1.82; NADP-MDH) was 1.6 times higher in rat heart under ischemia. NADP-MDH was purified from normal and ischemia-exposed rat myocardium. Using NADP-MDH purified enzyme preparations the values if Hill coefficient for oxaloacetate (1.83 ± 0.07 and 1.50 ± 0.10) and Km for NADPH (0.058 ± 0.003 and 0.096 ± 0.004 mM) were determined for the enzyme at norm and under ishemia respectively. Effects of Fe2+, Ca2+, Cu2+ ions, H2O2, oxidized and reduced glutathione, adenine nucleotides influence on functioning of NADP-MDH from rat heart at norm and under ischemic conditions have been investigated.
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Keywords: rats, myocardium, ischemia, free-radical processes, NADP-malate dehydrogenase
Citation:

Safonova, O. A., Popova, T. N., Matasova, L. V., Artyukhov, , V. G. (2005). Free radical processes intensity and regulation of cytoplasmic NADP-dependent malate dehydrogenase in rat cardiomyocytes at norm and under ischemia. Biomeditsinskaya khimiya, 51(3), 311-320.
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