Investigation of peroxide modification of low- and very low-density lipoproteins in patients with coronary heart disease by the method of fluorescent spectroscopy

   
Aidyraliev R.K.1 , Igemberdieva O.A.1, Dadabaev M.Kh.1, Murataliev T.M.1, Aitbaev K.A.1, Aldashev A.A.1

1. National Center of Cardiology and Internal Medicine, Kirghiz Ministry of Health
Section: Experimental/Clinical Study
DOI: 10.18097/pbmc20115701127      PubMed Id: 21516784
Year: 2011  Volume: 57  Issue: 1  Pages: 127-132
In the total fraction of low and very low density lipoproteins (LDL+VLDL) isolated from serum by precipitation in the presence of heparin-Mn the copper-induced lipid peroxidation was accompanied by accumulation of LPO products, a decrease ANS fluorescence intensity (FANS) and an increase in probe - cation DSP-6, a fluorescence intensity decrease of intrinsic in the ultraviolet area (Fuv) and an increase in the visible area (Fvis). The degree of lipoprotein modification was estimated by calculating the Fvis / Fuv and FDSP-6 / FANS ratio. Strong positive correlation was found between these ratios and concentration of thiobarbituric acid-reactive substances (TBARS) of LDL+VLDL samples isolated from sera of 49 donors and incubated at 37°С in the presence of 50 M CuSO4 during 0, 3 and 24 hr (Fvis / Fuv (r=0,75; p<0,001) and FDSP-6 / FANS (r=0,73; p<0,001)). Very strong positive correlation was also found between both fluorescent parameters Fvis / Fuv and FDSP-6 / FANS (r=0,95, p<0,001). Changes in the values of Fvis / Fuv, FDSP-6 / FANS, concentration of TBARS in 75 patients with documented coronary heart disease (CHD) and 49 apparently healthy donors were studied. No significant differences of these parameters in LDL+VLDL of patients with CHD and donors were found.
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Keywords: low-density lipoproteins, very low-density lipoproteins, lipid peroxidation, intrinsic fluorescence, fluorescent probes, coronary heart disease
Citation:

Aidyraliev, R. K., Igemberdieva, O. A., Dadabaev, M. Kh., Murataliev, T. M., Aitbaev, K. A., Aldashev, A. A. (2011). Investigation of peroxide modification of low- and very low-density lipoproteins in patients with coronary heart disease by the method of fluorescent spectroscopy. Biomeditsinskaya khimiya, 57(1), 127-132.
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