Interaction of rifampicin embedded in phospholipid nanoparticles with blood plasma lipoproteins

Sanzhakov M.A.1, Ipatova O.M.1, Prozorovskiy V.N.1, Medvedeva N.V.1, Torkhovskaya T.I.1

1. Orekhovich Institute of Biomedical Chemistry of Russian Academy of Medical Sciences
Section: Experimental Study
DOI: 10.18097/PBMC20146003348      PubMed Id: 25019397
Year: 2014  Volume: 60  Issue: 3  Pages: 348-353
The drug formulations of antituberculous remedy rifampicin in nanoparticles less than 30 nm based on soy phosphatidylcholine and sodium oleate was elaborated in Institute of Biomedical Chemistry. The distribution of rifampicin in blood plasma fractions after incubation with this formulation and with free rifampicin was studied. This goal was stimulated by the literature data about activation of macrophages LDL receptors in cases of M. tuberculosis infection. Plasma was incubated 30 min with free rifampicin or rifampicin encapsulated into the nanoformulation followed by ultracentrifugation and subsequent rifampicin determination by HPLC in lipoprotein fractions. In the case of free rifampicin it appeared mainly in the plasma protein fraction and in HDL (41% and 38%, correspondentely). But after incubation of rifampicin in nanoparticles the drug redistribution was observed. Its proportion in these factions decreased 2-3-fold, and it was found mainly in LDL (60% as compared with 21% for free rifampicin). The increased association of rifampicin encapsulated into phospholipid nanoparticles with LDL is considered as facilitating factor for macrophages delivery and thus for antituberculosis efficiency as well
Download PDF:  
Keywords: rifampicin, tuberculosis treatment, phospholipids nanoparticles, low density lipoproteins

Sanzhakov, M. A., Ipatova, O. M., Prozorovskiy, V. N., Medvedeva, N. V., Torkhovskaya, T. I. (2014). Interaction of rifampicin embedded in phospholipid nanoparticles with blood plasma lipoproteins. Biomeditsinskaya Khimiya, 60(3), 348-353.
 2023 (vol 69)
 2022 (vol 68)
 2021 (vol 67)
 2020 (vol 66)
 2019 (vol 65)
 2018 (vol 64)
 2017 (vol 63)
 2016 (vol 62)
 2015 (vol 61)
 2014 (vol 60)
 2013 (vol 59)
 2012 (vol 58)
 2011 (vol 57)
 2010 (vol 56)
 2009 (vol 55)
 2008 (vol 54)
 2007 (vol 53)
 2006 (vol 52)
 2005 (vol 51)
 2004 (vol 50)
 2003 (vol 49)