Extracorporeal treatment of nucleoprotein disposal disorders using the systemic lupus erythematosus model: evaluation of efficacy and safety in a short-term simulated experiment
1. Research Institute for Clinical and Experimental Rheumatology RAMS 2. Volgograd State Medical University, Volgograd, Russia 3. Research Institute for Clinical and Experimental Rheumatology RAMS, Volgograd, Russia
Efficacy and safety of the extracorporeal blood perfusion through DNase I- and C1q-containing magnetic beads have been evaluated using the experimental model simulating the nucleoprotein disposal disorders in systemic lupus erythematosus (SLE). The study was performed using 20 rats in which the essential impairments of nucleoprotein catabolism typical for SLE were modeled. The animals were randomized into the experimental group and the placebo perfusion control group. Rats of the experimental group were characterized by the statistically significant reduction of increased levels of circulating immune complexes and plasma DNA as well as diminished levels of plasma creatinine and kidney IgG deposition as compared with placebo controls. During short-term experiment there were neither animal deaths nor substantial blood cell destruction and hepatotoxicity signs.
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Keywords: systemic lupus erythematosus, extracorporeal therapy, C1q, DNase I, biosimulation
Citation:
Trofimenko A.S., Gontar I.P., Paramonova O.V., Simakova E.S., Zborovskaya I.A. (2015) Extracorporeal treatment of nucleoprotein disposal disorders using the systemic lupus erythematosus model: evaluation of efficacy and safety in a short-term simulated experiment. Biomeditsinskaya Khimiya, 61(5), 622-627.
Trofimenko A.S. et al. Extracorporeal treatment of nucleoprotein disposal disorders using the systemic lupus erythematosus model: evaluation of efficacy and safety in a short-term simulated experiment // Biomeditsinskaya Khimiya. - 2015. - V. 61. -N 5. - P. 622-627.
Trofimenko A.S. et al., "Extracorporeal treatment of nucleoprotein disposal disorders using the systemic lupus erythematosus model: evaluation of efficacy and safety in a short-term simulated experiment." Biomeditsinskaya Khimiya 61.5 (2015): 622-627.
Trofimenko, A. S., Gontar, I. P., Paramonova, O. V., Simakova, E. S., Zborovskaya, I. A. (2015). Extracorporeal treatment of nucleoprotein disposal disorders using the systemic lupus erythematosus model: evaluation of efficacy and safety in a short-term simulated experiment. Biomeditsinskaya Khimiya, 61(5), 622-627.
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