Investigation of the surface layer of 3D-matrices for tissue engineering

Chernonosova V.S.1 , Kvon R.I.2, Kiseleva E.V.3, Stepanova A.O.1, Laktionov P.P.1

1. Institute of Chemical Biology and Fundamental Medicine Siberian Branch of the Russian Academy of Science, Novosibirsk, Russia; Meshalkin Siberian Federal Biomedical Research Center, Novosibirsk, Russia
2. Boreskov Institute of Catalysis Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia
3. Institute of Cytology and Genetics Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia
Section: Experimental Study
DOI: 10.18097/PBMC20176301032      PubMed Id: 28251949
Year: 2017  Volume: 63  Issue: 1  Pages: 32-38
Electrospinning is a convenient and promising manufacturing method a variety of materials for tissue engineering. 3D matrices fabricated by electrospinning from solutions of polycaprolactone with human serum albumin or gelatin in 1,1,1,3,3,3-hexafluoroisopropanol were studied. The microstructure of the 3D matrices and surface of the fibers were investigated using scanning electron microscopy. Protein distribution in the surface layer was studied by modification of protein amino groups with N-(2-hydroxyethyl)phenazine and X-ray photoelectron spectroscopy. It was shown, that concentration of the proteins in the surface layer of fibers exceeded their concentration in the initial electrospun solution up to 12 times and the surface layer was enriched in the protein inversely to the concentration of the protein in solution. The minor part of the proteins was released from fibers during first 30-60 min after swelling in water. Treatment of matrices with proteinase K hydrolyzed about 1/3 of the surface exposed human serum albumin. Thus, both methods can be used to study the surface content of the materials produced by electrospinning from blends of synthetic and natural polymers, however X-ray photoelectron spectroscopy appears to be more convenient and informative.
Download PDF:  
Keywords: 3D-matrices, synthetic and natural polymers, electrospinning, X-ray photoelectron spectroscopy, chemical modification

Chernonosova, V. S., Kvon, R. I., Kiseleva, E. V., Stepanova, A. O., Laktionov, P. P. (2017). Investigation of the surface layer of 3D-matrices for tissue engineering. Biomeditsinskaya Khimiya, 63(1), 32-38.
This paper is also available as the English translation: 10.1134/S1990750817020020
 2024 (vol 70)
 2023 (vol 69)
 2022 (vol 68)
 2021 (vol 67)
 2020 (vol 66)
 2019 (vol 65)
 2018 (vol 64)
 2017 (vol 63)
 2016 (vol 62)
 2015 (vol 61)
 2014 (vol 60)
 2013 (vol 59)
 2012 (vol 58)
 2011 (vol 57)
 2010 (vol 56)
 2009 (vol 55)
 2008 (vol 54)
 2007 (vol 53)
 2006 (vol 52)
 2005 (vol 51)
 2004 (vol 50)
 2003 (vol 49)