Voprosy meditsinskoi khimii (ISSN 0042-8809)

Synthesis and study of the properties of water-insoluble products of the interaction of urease and cellulose derivatives

   
Fesenko R.I., Artemova I.V., Virnik A.D., Rogovin Z.A., Iakovlev V.A.
PubMed Id: 1119115
Year: 1975 vol: 21  issue:1  pages: 82-84
Abstract: As a result of reaction of urease with graft copolymer of cellulose and polyglycidyle methacrylate or with carboxymethyl cellulose, products were synthesized, containing about 2% of chemically bound urease. Binding with the cellulose derivatives was accompanied by about two-fold decrease in urease activity. Carboxymethyl cellulose-urease and polyglycidile methacrylate-cellulose-urease might be repeatedly (for 30 cycles) used for hydrolysis of urea; the enzymatic activity of the first compound did not change, but of the second one--was decreased by 30%. Activity of the compounds was not changed after storage within 2 months in 0.1 M phosphate buffer, pH 7.0 at 4 degrees C.
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Reference: Fesenko R.I., Artemova I.V., Virnik A.D., Rogovin Z.A., Iakovlev V.A., Synthesis and study of the properties of water-insoluble products of the interaction of urease and cellulose derivatives, Voprosy meditsinskoi khimii, 1975, vol: 21(1), 82-84.
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