Voprosy meditsinskoi khimii (ISSN 0042-8809)

Study of mechanisms of regulation of disulphide reductase in mouse liver

Kulinsky V.I., Kolesnichenko L.S.
PubMed Id: 174302
Year: 1975 vol: 21  issue:3  pages: 286-290
Abstract: An increase in activity of disulphide reductase system (DRS) in supernatant of liver tissue was caused by 3',5'-AMP, ATP, GTP, UTP, Mg2+, Ca2+, EDTA, protamine, noradrenaline and F-. The effect was connected with arsenite resistant fraction of DRS. After rapid homogenization the effect of noradrenaline disappeared and the effects of ATP, GTP, UTP and Ca2+ were distinctly decreased. Treatment with adsorbents prevented the effects of 3',5,-AMP, ATP and EDTA and markedly decreased the effects of protamine and Mg2+. A protein inhibitor of protein kinase prevented completely the activation of DRS with 3',5'-AMP, ATP, GTP, UTP and noradrenaline and distinctly decreased the effect of protamine, Mg2+ and Ca2+ but did not alter the influence of EDTA. Addition of 2',3'-AMP blocked the effect of 3',5-AMP, ATP and Mg2+ but did not influence the effect of protamine and EDTA. The data obtained suggest that protein kinase participated in activation of DRS by most of the regulators.
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Reference: Kulinsky V.I., Kolesnichenko L.S., Study of mechanisms of regulation of disulphide reductase in mouse liver, Voprosy meditsinskoi khimii, 1975, vol: 21(3), 286-290.