Voprosy meditsinskoi khimii (ISSN 0042-8809)

Fluorimetric ultramicrodetermination of the dansyl derivatives of cyclic 3'5-AMP by thin-layer chromatography

   
Jaliashvili T.A., Chikvaidze V.H.
PubMed Id: 175567
Year: 1975 vol: 21  issue:4  pages: 429-432
Abstract: A new fluoriemtric ultramicromethod was developed for quantiative determination of cyclic 3',5'-AMP as derivative of dansyl chloride using silica gel thin-layer chromatography. In presence of dansyl chloride cyclic 3',5'-AMP formed a fluorescent complex. A spot of dansyl derivative of cyclic 3',5'-AMP was photographed on a reversible colour film, after which the colour intensity of the spot on the film was determined directly by a special densitometer. Content of cyclic 3',5'-AMP was studied in different regions of rat brain. The in fluence of some psychotropic drugs on the level of cyclic 3',5'-AMP was studied using this method. The method was highly sensitive and reproducible and enabled to determine 10(-10) -- 10(-11) M cyclic 3',5'-AMP.
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Reference: Jaliashvili T.A., Chikvaidze V.H., Fluorimetric ultramicrodetermination of the dansyl derivatives of cyclic 3'5-AMP by thin-layer chromatography, Voprosy meditsinskoi khimii, 1975, vol: 21(4), 429-432.
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