Voprosy meditsinskoi khimii (ISSN 0042-8809)

Preparation of labelled DNA from animal tissues

Rusinova G.G., Rogacheva S.A.
PubMed Id: 1216771
Year: 1975 vol: 21  issue:5  pages: 542-548
Abstract: Incorporation of 3H-thymidine into cells of bone marrow, spleen and intestinal mucosa was studied in rats with anemia, caused by administration of phenyl hydrazine-HCl. Optimal duration for administration of the labelled precursor was determined and the doses of 3H-thymidine required to produce 3H-DNA with the definite specific radioactivity were established. The isolation of the labelled preparations of DNA from rat spleen after incorporation of 2-2.5 muCi of 3H-thymidine is described; their physico-chemical properties were studied. The specific radioactivity of 3H-DNA was equal to 5-10(6)--10-10(6) disintegrations per 1 min per 1 mg.
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Reference: Rusinova G.G., Rogacheva S.A., Preparation of labelled DNA from animal tissues, Voprosy meditsinskoi khimii, 1975, vol: 21(5), 542-548.