Voprosy meditsinskoi khimii (ISSN 0042-8809)

A modified method for phosphoribosyl pyrophosphate synthesis

   
Filippova V.N., Filanovskaya L.I., Blinov M.N.
PubMed Id: 175573
Year: 1975 vol: 21  issue:6  pages: 663-665
Abstract: A modified method for synthesis of phosphoribosyl pyrophosphate (PRPP) from ribose-5-phosphate and ATP in presence of ribose phosphate pyrophosphokinase (RPPPK) was developed. RPPPK was isolated from extracts of acetone powder of rabbit liver tissue, using alcohol fractionation of proteins. The protein fraction, containing RPPPK, was isolated at 35% saturation with 96% ethyl alcohol. The identification of the synthesized preparation of PRPP was carried out in enzymatic reaction with adenine- and inosine phosphoribosyl transferases, using labelled adenine and guanine. The obtained product of reaction (AMP and GMP) were precipitated with lantane chloride.
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Reference: Filippova V.N., Filanovskaya L.I., Blinov M.N., A modified method for phosphoribosyl pyrophosphate synthesis, Voprosy meditsinskoi khimii, 1975, vol: 21(6), 663-665.
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