Voprosy meditsinskoi khimii (ISSN 0042-8809)

Messenger RNA metabolism in E. coli under amino acid starvation conditions

Arbuzov V.A.
PubMed Id: 799868
Year: 1976 vol: 22  issue:2  pages: 194-203
Abstract: In E. coli Hfr Kavalli (met-) turnover of mRNA was studied in normal state and in methionine deficiency. This strain of E. coli was shown to have the 'loose' type of regulation of RNA synthesis (RC-). Without methionine in E. coli Hfr the rate of the protein synthesis was about 7-8% of that found in the control culture. In studies of the total mRNA turnover the half-life of matrices was equal to 2 min at 37degree, if a medium did not contain methionine; the same value was observed in the control culture. At the same time in methionine starvation an increase in amount of rapidly labelled RNA was observed; the RNA remained stable under simultaneous effect of actinomycin D. This rapidly labelled, stable RNA was the messenger-RNA, associated with cytoplasmic membranes. The hypothesis is advanced that membrane-associated and cytoplasmic RNA are those two types of matrices, which differed in their stability when the protein synthesis was inhibited.
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Reference: Arbuzov V.A., Messenger RNA metabolism in E. coli under amino acid starvation conditions, Voprosy meditsinskoi khimii, 1976, vol: 22(2), 194-203.