Voprosy meditsinskoi khimii (ISSN 0042-8809)

Adenylate deaminase of the liver mitochondria in normal state and in alcoholic intoxication

   
Isakhanian G.D., Gorkin V.Z.
PubMed Id: 1035994
Year: 1976 vol: 22  issue:3  pages: 380-388
Abstract: AMP-deaminases were isolated and partially purified from subfractions of soluble mitochondrial proteins of rat liver under normal conditions and in ethanol intoxication. Repeated freezing and thawing of the mitochondrial fractions from liver of rats, which were treated with ethanol (1 ml of 32% solution daily for 7 days, intraperitoneally), liberated into the subfraction of soluble mitochondrial proteins significantly less AMP-deaminases, as compared with the control animals. The enzyme preparations obtained from intoxicated and intact animals were quite similarly inactivated by controlled heating, deaminated at similar rates AMP, ADP, FAD and some other nitrogenous compounds (but did not deaminate adenosine and some structural analogues of AMP). However, an inhibitory effect of the structural analogues of AMP and of nucleosides was significantly higher towards the AMP-deaminase from healthy rats as compared with the corresponding enzyme preparations obtained from the ethanol-treated animals. The increase in velocity of enzymatic AMP deamination in the subfraction of soluble mitochondrial proteins apparently does not represent a suitable target for possible therapeutic approaches to control the phenomenon, observed in the experimental ethanol intoxication, of stimulation of the deaminating activity in total mitochondrial fraction of rat liver.
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Reference: Isakhanian G.D., Gorkin V.Z., Adenylate deaminase of the liver mitochondria in normal state and in alcoholic intoxication, Voprosy meditsinskoi khimii, 1976, vol: 22(3), 380-388.
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