Voprosy meditsinskoi khimii (ISSN 0042-8809)

Method of gradient chromatography for the isolation of purified lipid fractions from nerve tissue in studies using radioactive carbon, C14

   
Taranova N.P.
PubMed Id: 1027245
Year: 1976 vol: 22  issue:4  pages: 567-569
Abstract: A method is developed for separation of lipids from nervous tissue by thin-layer chromatography in silica gel. Cholesterol and 'minor' lipids were removed from the start by the first chromatography in the system ether-benzol-ethanol-acetic acid. Phospholipids and galactolipids, which remained at the start, were separated by the repeated chromatography in the system chloroform-methanol-NH4OH. The purified fractions of cholesterol, cerebrosides, sulfatides and the total fraction of phospholipids were obtained from 100-150 mg of nervous tissue in amounts, sufficient for estimation of their specific radioactivity in experiments with 14C.
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Reference: Taranova N.P., Method of gradient chromatography for the isolation of purified lipid fractions from nerve tissue in studies using radioactive carbon, C14, Voprosy meditsinskoi khimii, 1976, vol: 22(4), 567-569.
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