Voprosy meditsinskoi khimii (ISSN 0042-8809)

Fractionation of DNA on unmodified cellulose

Kotin A.M., Teriukova N.P.
PubMed Id: 1014486
Year: 1976 vol: 22  issue:5  pages: 712-716
Abstract: Not only separation of native, two-stranded DNA from denatured molecules but more distinct fractionation of single-stranded DNA was demonstrated on unmodified powdery and microgranular cellulose. Denatured DNA, absorbed on cellulose, was eluted from a column as two asymmetric peaks by 0,12 M and 0.05 M NaCl (for I and II peaks, respectively). Absorption and fractionation of denatured DNA and poly-A containing RNA on unmodified cellulose was apparently achieved on the basis of the same principles.
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Reference: Kotin A.M., Teriukova N.P., Fractionation of DNA on unmodified cellulose, Voprosy meditsinskoi khimii, 1976, vol: 22(5), 712-716.