Voprosy meditsinskoi khimii (ISSN 0042-8809)

New variant of glucose-6-phosphate dehydrogenase (G-6-PD 'Kaluga') from erythrocytes of a patient with chronic nonspherocytic hemolytic anemia

   
Shatskaia T.L., Krasnopol'skaia K.D., Idel'son L.I.
PubMed Id: 17221
Year: 1976 vol: 22  issue:6  pages: 764-768
Abstract: Properties of a new form of glucose-6-phosphate dehydrogenase (G6PD) from erythrocytes, called 'Kaluga', are described. The enzyme was isolated from erythrocytes of a patient with chronic non-spherocytic hemolytic anemia; in the blood cells 20% of the G6PD activity was maintained as compared with normal state. The partially purified enzyme was shown to be unstable in electrophoresis, it possessed a biphase type of pH-optima; Km value for NADP was decreased for glucose-6-phosphate Km value was normal. The thermostability of G6PD was normal at 46 within 1 hr.
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Reference: Shatskaia T.L., Krasnopol'skaia K.D., Idel'son L.I., New variant of glucose-6-phosphate dehydrogenase (G-6-PD 'Kaluga') from erythrocytes of a patient with chronic nonspherocytic hemolytic anemia, Voprosy meditsinskoi khimii, 1976, vol: 22(6), 764-768.
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