Voprosy meditsinskoi khimii (ISSN 0042-8809)

Fast and highly-sensitive method of quantitative determination of carnosine and anserine in animal tissues

   
Tkachuk V.A., Malinovskaia K.I.
PubMed Id: 857404
Year: 1977 vol: 23  issue:1  pages: 127-131
Abstract: A method is described for separation of carnosine and anserine and of the amino acids constituting them by means of thin-layer chromatography. For quantitative estimation the amino acids and dipeptides were treated with 7-chlorine-4-nitrobenzeno-2-hydroxy-1,3-diazol (NBD-chloride) and subsequently separated by chromatography on silica gel and estimated by absorption at 456-470 nm. Systems of solvents were developed for separation of both free and chemically modified amino acids and dipeptides; optimal conditions were chosen for preparation of tissue extracts and their treatment with NBD-chloride. By this method carnosine and anserine could be stimated in extract from 0.02-0.2 mg of muscular tissue. The time required for the analyses was about 3-5 hrs.
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Reference: Tkachuk V.A., Malinovskaia K.I., Fast and highly-sensitive method of quantitative determination of carnosine and anserine in animal tissues, Voprosy meditsinskoi khimii, 1977, vol: 23(1), 127-131.
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