Voprosy meditsinskoi khimii (ISSN 0042-8809)

Native and desensitized forms of L-type pyruvate kinase from rabbit kidney cortex

   
Kozhevnikova K.A.
PubMed Id: 595502
Year: 1977 vol: 23  issue:5  pages: 658-661
Abstract: A time-consuming procedure of isolation of pyruvate kinase isoenzymes from rabbit kidney cortex (more than 5 hrs) at 0-2 degrees led to obtaining of a desensitized form of 'L' type, resembling the 'M3' type of the enzyme from sceletal muscle. Rapid isolation of pyruvate kinase 'L' type (within about 2.5 hrs) at 4-6 degrees provided the isoenzyme in the active (allosteric) form.
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Reference: Kozhevnikova K.A., Native and desensitized forms of L-type pyruvate kinase from rabbit kidney cortex, Voprosy meditsinskoi khimii, 1977, vol: 23(5), 658-661.
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