Voprosy meditsinskoi khimii (ISSN 0042-8809)

Effect of S-nucleosyl-L-homocysteines on the activity of DNA methylases

   
Nikol'skaia I.I., Lopatina N.G., Rekunova V.N., Iurkevich A.M., Debov S.S.
PubMed Id: 351945
Year: 1978 vol: 24  issue:2  pages: 252-255
Abstract: It was studied effect of S-adenosyl, -uridyl, -citidyl and -inosyl homocysteines on activity of bacterial adenine and cytosine methylases from E. coli CK as well as on guanine methylase specific for DDVI phage. S-adenosyl homocysteine was shown to be the strong inhibitor of methylation; 10 micrometer of the substance inhibited all the enzymes studied by 98--99%. Use of total enzymatic preparations did not enable to find a difference in affinity of S-uridyl, -citidyl, and -inosyl homocysteines to various DNA methylases studied. All these preparations inhibited DNA methylases by 55--65%. Increase in concentration of inhibitor up to 20 micrometer did not elevate the inhibitory effect. Action of S-nucleosyl homocysteines did not depend on the type of acceptory DNA.
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Reference: Nikol'skaia I.I., Lopatina N.G., Rekunova V.N., Iurkevich A.M., Debov S.S., Effect of S-nucleosyl-L-homocysteines on the activity of DNA methylases, Voprosy meditsinskoi khimii, 1978, vol: 24(2), 252-255.
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