Voprosy meditsinskoi khimii (ISSN 0042-8809)

Determination of hexokinase isoenzymes in different tissues

Shurda G.G., Panin L.E.
PubMed Id: 758095
Year: 1978 vol: 24  issue:2  pages: 282-285
Abstract: A modified method is described for estimation of hexokinase isoenzymes by polyacrylamide gel electrophoresis. The best conditions for detection of hexokinase activity were developed using immobilization of glucose-6-phosphate dehydrogenas: in gel, utilization of riboflavin as a catalyst of polymerization and stabilization by glucose. The method is simple, highly reproducible, comparatively rapid and economically reasonable.
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Reference: Shurda G.G., Panin L.E., Determination of hexokinase isoenzymes in different tissues, Voprosy meditsinskoi khimii, 1978, vol: 24(2), 282-285.