Voprosy meditsinskoi khimii (ISSN 0042-8809)

Thiamine- and thiamine diphosphate-binding capacity of the hyaloplasmic fraction of rat liver

   
Vinogradov V.V., Strumilo S.A., Mandrik K.A., Ostrovsky Yu.M.
PubMed Id: 664470
Year: 1978 vol: 24  issue:3  pages: 383-389
Abstract: Hyaloplasmic fraction of rat liver tissue did not bind 14C-thiamin and 14C-thiamin diphosphate in vitro as shown by means of gel filtration and equilibrium dialysis. All the thiamin and 2/3 of thiamin diphosphate were in vivo in free state or in a state unbound with proteins. Hyaloplasm lost readily the endogenous thiamin diphosphate during dialysis. Endogenous thiamin diphosphate (about 1/3 of the initial content), resistent to dialysis and gel filtration, was apparently included in transketolase in hyaloplasmic fraction of rat liver tissue.
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Reference: Vinogradov V.V., Strumilo S.A., Mandrik K.A., Ostrovsky Yu.M., Thiamine- and thiamine diphosphate-binding capacity of the hyaloplasmic fraction of rat liver, Voprosy meditsinskoi khimii, 1978, vol: 24(3), 383-389.
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