Voprosy meditsinskoi khimii (ISSN 0042-8809)

Method of measuring binding constants and the number of specific cyclic AMP receptor sites in tissue extracts

   
Gorbachevskaia L.V.
PubMed Id: 664475
Year: 1978 vol: 24  issue:3  pages: 423-427
Abstract: A method for evaluation of binding constants and of amount of cAMP binding sites in crude tissue extracts was developed. The method is based on equilibrium binding of 3H-cAMP by proteins with subsequent ultrafiltration. Hydrolysis of cAMP and its unspecific sorption by proteins were eliminated under the conditions selected. Rat spleen cytosole contained 3.57 +/- 0.34 pmol of cAMP binding sites per mg of protein with dissociation constant of protein-cAMP complex (1.68 +/- 0.28).10(-8) M. As shown by studies on kinetics, binding constants and specificity of binding, the method permitted to evaluate quantitatively cAMP-dependent protein kinases in crude tissue extracts and to estimate their affinity to cAMP.
Download PDF:
Reference: Gorbachevskaia L.V., Method of measuring binding constants and the number of specific cyclic AMP receptor sites in tissue extracts, Voprosy meditsinskoi khimii, 1978, vol: 24(3), 423-427.
References
 1985(Vol:31)
 1984(Vol:30)
 1983(Vol:29)
 1982(Vol:28)
 1981(Vol:27)
 1980(Vol:26)
 1979(Vol:25)
 1978(Vol:24)
 1977(Vol:23)
 1976(Vol:22)