Voprosy meditsinskoi khimii (ISSN 0042-8809)

Characteristics of human plasminogen activator and of a fraction of fibrinolytically active plasma enriched with this enzyme

   
Platonova T.K., Petrenko O.A.
PubMed Id: 156462
Year: 1979 vol: 25  issue:3  pages: 302-307
Abstract: An activator of plasminogen with specific activity 203 AU/mg was isolated from blood plasma with fibrinolytic activity, obtained from blood of suddenly decreased patients. Specific activity of the plasminogen activator exceeded 88-fold the activity of the initial blood plasma. The protein was identified with a glycoprotein, similar to beta-globulin; its molecular weight was 70,000 as shown by gel filtration; the isoelectric point was at pH 6.2. The plasminogen activator remained stable after heating up to 50 degrees. Effects of pH in an incubation media and of cations on the activity of the plasminogen activator were studied. A fraction containing the fibrinolytic activity and enriched with plasminogen activator was obtained from the blood plasma after fractionation at low temperature with ethanol at definite pH value. The specific fibrinolytic activity in the fraction exceeded 17.6-fold the activity of blood plasma. The fraction exhibited high thrombolytic and antithrombin activities in vitro. It was similar to streptase and streptokinase preparations in its throm-bolytic effect. Relative species specificity was found in studies of the fibrinolytic and antithrombin effects of the fraction containing fibrinolytic activity.
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Reference: Platonova T.K., Petrenko O.A., Characteristics of human plasminogen activator and of a fraction of fibrinolytically active plasma enriched with this enzyme, Voprosy meditsinskoi khimii, 1979, vol: 25(3), 302-307.
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