Voprosy meditsinskoi khimii (ISSN 0042-8809)

Isolation of the plasma membranes of fat cells without using collagenase

   
Bezdrobnyj I.V., Evdokimova N.Yu.
PubMed Id: 222073
Year: 1979 vol: 25  issue:3  pages: 354-359
Abstract: A method is described for isolation of plasmatic membranes of rat fatty cells immediately from fatty tissue without the treatment with collagenase. Homogenization of fatty tissue was carried out in large volumes of buffered sucrose and EDTA at room temperature followed by sucrose density gradient centrifugation. The preparations obtained exhibited high specific activity of the marker enzyme of plasmatic membranes [5'-nucleotidase and K+, Na+-ATPase], as well as high ability for specific binding of insulin
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Reference: Bezdrobnyj I.V., Evdokimova N.Yu., Isolation of the plasma membranes of fat cells without using collagenase, Voprosy meditsinskoi khimii, 1979, vol: 25(3), 354-359.
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