Voprosy meditsinskoi khimii (ISSN 0042-8809)

Detection of plasmids in the cells of E. coli CK

   
Anikeĭcheva N.V., Aleksandrova S.S., Debov S.S., Nikolskaya I.I.
PubMed Id: 382626
Year: 1979 vol: 25  issue:4  pages: 395-397
Abstract: Superspiralized coiled plasmide DNA were found in cells E. coli CK. One of plasmides pEco CK-1 had a size 21.2 micron that corresponded to molecular mass 43.7 megadaltons, another plasmide pEco CK-2 with the size 14.2 micron had a molecular weight 29.2 megadaltons. Effective restriction was observed in cross titration using phage PBV-I.CK for E. coli strains carrying R II plasmide and phage PBV-I.R II for E. coli CK cells. The data obtained suggest that the Eco CK type with DNA of host specificity is distinct from hsp II type due to presence of R II and N-15 plasmides.
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Reference: Anikeĭcheva N.V., Aleksandrova S.S., Debov S.S., Nikolskaya I.I., Detection of plasmids in the cells of E. coli CK, Voprosy meditsinskoi khimii, 1979, vol: 25(4), 395-397.
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