Voprosy meditsinskoi khimii (ISSN 0042-8809)

Isoenzymes of mitochondrial thymidine kinase from normal and proliferating rat liver tissues

   
Silaeva S.A., Danilova N.I.
PubMed Id: 473680
Year: 1979 vol: 25  issue:4  pages: 415-419
Abstract: Isoenzyme composition of thymidine kinase was studied in submitochondrial fractions of liver tissue with various proliferative activity (intact, regenerating livers and Zhaidel ascites hepatoma) using polyacrylamide gel disc electrophoresis. Three zones, corresponding to proteins with Rf 0.1-0.2 (I) Rf 0.5-0.55 (II) and Rf 0.85-0.87 (III) and exhibiting thymidine kinase activity, were found in fractions of cytoplasmic and mitochondrial matrix proteins from resting and proliferating rat liver tissues. In fractions of outer and inner mitochondrial membranes three = zones of the enzymatic activity were also observed but two of them did not coincide in the Rf value with the thymidine kinase isoenzymes from cytoplasmic fraction and mitochondrial matrix: I Rf 0.1-0.16, II Rf 0.35-0.4 and III Rf 0.62-0.68. Redistribution of the enzymatic activity between thymidine kinase isoenzymes occurred in conversion of liver tissue from the resting state to increase proliferation. In these cases slowly migrating enzymatic fraction (Rf 0.1-0.2) was activated in mitochondrial matrix and membranes; formation of TMP, catalyzed by isoenzymes with fast mobility (Rf 0.5-0.55 in matrix and Rf 0.62-0.68 in membrane fractions of mitochondrial which are typical for intact liver tissue, was decreased, respectively.
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Reference: Silaeva S.A., Danilova N.I., Isoenzymes of mitochondrial thymidine kinase from normal and proliferating rat liver tissues , Voprosy meditsinskoi khimii, 1979, vol: 25(4), 415-419.
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