Voprosy meditsinskoi khimii (ISSN 0042-8809)

Identification of the amino acids participating in interaction with DNA in the makeup of a viral nucleoprotein

   
Skliadneva V.B., Chekanovskaia L.A., Tikhonenko T.I.
PubMed Id: 473684
Year: 1979 vol: 25  issue:4  pages: 436-439
Abstract: A method is described for identification of amino acids participating in covalent DNA-protein cross-links formed in the course of modification of native CD phage by soldium bisulfite. The method is based on estimation of increased content of free amino acids, formed after treatment of the isolated cross-link products using equimolar mixture of O-methyl hydroxylamine and sodium bisulfite. Thin layer chromatography on the plates Fixion 50 x 8 was used for comparative semi-quantitative analysis of amino acids.
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Reference: Skliadneva V.B., Chekanovskaia L.A., Tikhonenko T.I., Identification of the amino acids participating in interaction with DNA in the makeup of a viral nucleoprotein, Voprosy meditsinskoi khimii, 1979, vol: 25(4), 436-439.
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