Voprosy meditsinskoi khimii (ISSN 0042-8809)

A method for estimation of α1-antitrypsin and of α2-macroglobulin in human blood serum (plasma) in normal state and under some pathological conditions

   
Nartikova N.F., Paskhina T.S.
PubMed Id: 89758
Year: 1979 vol: 25  issue:4  pages: 494-499
Abstract: A modified spectrophotometric method is developed for simultaneous estimation of α1-antitrypsin and α2-macroglobulin in human blood serum (plasma); the method is based on dissimilar interaction of these inhibitors with trypsin in the systems with a low molecular substrate N-α-benzoyl-1-arginine ethyl ester. α1-Antitrypsin was estimated by inhibition of the arginine esterase activity of trypsin in a mixture containing human blood serum diluted 50-fold. α2-Macroglobulin was estimated by maintained arginine esterase activity of the trypsin-α2-macroglobulin complex, formed after interaction of an excess of trypsin with blood serum, diluted 10-fold and after subsequent inactivation of free, unbound with α2-macroglobulin, trypsin by treatment with the soy bean inhibitor of trypsin. α1-Antitrypsin and α2-macroglobulin were estimated by means of the method described in blood serum of healthy persons and in patients with burns or with carcinoma of pancreas. The method enables to estimate two main inhibitors of blood plasma proteinases in a small volume of blood serum (0.1 ml) very rapidly and specifically using commercially available substrate; the method might be recommended for routine clinical analysis.
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Reference: Nartikova N.F., Paskhina T.S., A method for estimation of α1-antitrypsin and of α2-macroglobulin in human blood serum (plasma) in normal state and under some pathological conditions , Voprosy meditsinskoi khimii, 1979, vol: 25(4), 494-499.
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