Voprosy meditsinskoi khimii (ISSN 0042-8809)

Polyamine oxidative deamination in hepatomas with varying growth rates

   
Siatkin S.P., Berezov T.T.
PubMed Id: 115153
Year: 1979 vol: 25  issue:5  pages: 611-617
Abstract: Rates of oxidative deamination of polyamines were studied in rapidly growing hepatomas G-22 and G-27, in slowly growing hepatomas G-60, G-61, G-48, G-46 as well as in liver tissue of tumor-bearing animals and animals treated with nitrose piperidine. Diamine oxidase activity was not found in rapidly growing hepatomas. Treatment with pyridoxal-phosphate did not restore the diamine oxidase activity in hepatomas G-27, but distinctly increased the latter in the liver tissue of the tumor-bearing animals up to the level of the enzymatic activity found in liver tissue of the intact animals. On the contrary, high concentrations of pyridoxal-phosphate (above 0.02 mg) inhibited the diamine oxidase activity in liver tissue of the impaired and intact animals. The enzymatic activity was markedly decreased in slowly growing hepatomas G-60, G-61, G-48 and G-46 as compared with the activity in liver tissue of tumor-bearing animals. Oxidation of all the substrates used could be measured using hepatoma G-60, putrescine and spermidine - for hepatoma G-61, but only putrescine - for hepatoma G-48. No of the substrates used was deaminated by hepatoma G-46. Four-fold decrease in the diamine oxidase activity was observed during malignization of liver cells induced by nitrose piperidine. The diamine oxidase was mainly localized in the postmitochondrial fraction of hepatocytes
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Reference: Siatkin S.P., Berezov T.T., Polyamine oxidative deamination in hepatomas with varying growth rates, Voprosy meditsinskoi khimii, 1979, vol: 25(5), 611-617.
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