Voprosy meditsinskoi khimii (ISSN 0042-8809)

Modification of a method of determining 15N in biological material

   
Pisarenko O.I., Minkovskii E.B., Studneva I.M., Merimson V.G., Rozynov B.V.
PubMed Id: 516541
Year: 1979 vol: 25  issue:6  pages: 782-786
Abstract: A method is described for preparation of nitrogen samples and estimation of 15N concentration in free amino acids and urea, isolated from heart muscle, using mass-spectometric analysis. Components of the tissue extracts were separated at the preparative scale; amino acids and urea were desalted by means of ion exchange chromatography. CHN-analyzer was used to measure content of nitrogen in urea and amino acids. The method required about 100 micrograms of nitrogen for the isotope analysis.
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Reference: Pisarenko O.I., Minkovskii E.B., Studneva I.M., Merimson V.G., Rozynov B.V., Modification of a method of determining 15N in biological material, Voprosy meditsinskoi khimii, 1979, vol: 25(6), 782-786.
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