Voprosy meditsinskoi khimii (ISSN 0042-8809)

Determination of somatotropin and prolactin by dodecyl sulfate polyacrylamide gel electrophoresis

   
Shostak I.N., Rozhko O.T., Martynenko F.P.
PubMed Id: 7368631
Year: 1980 vol: 26  issue:1  pages: 71-75
Abstract: Definite separation of somatotropin and prolactin using sodium dodecylsulphate polyacrylamide gel electrophoresis takes place in continuous systems with pH 7.0-7.2 containing acetate or chloride monovalet anions; substitution of the ions by polyvalent phosphate or borate anions interfered with the separation. The hormones precipitated by trichloracetic acid may be separated and quantitatively determined after dissolving in the weak alkaline solutions. The hormones are distinctly separated from serum proteins and may be quantitatively determined in media containing up to 15% of serum.
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Reference: Shostak I.N., Rozhko O.T., Martynenko F.P., Determination of somatotropin and prolactin by dodecyl sulfate polyacrylamide gel electrophoresis, Voprosy meditsinskoi khimii, 1980, vol: 26(1), 71-75.
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