Voprosy meditsinskoi khimii (ISSN 0042-8809)

Influence of S-methylmethionine on the metabolism of nucleic acids in Escherichia coli

   
Lebenka A.Yu., Rachkus I.A., Kanopkaite S.I.
PubMed Id: 6169200
Year: 1981 vol: 27  issue:2  pages: 207-210
Abstract: The influence of S-methylmethionine (SMM) on metabolism of nucleic acids in E. coli MRE-600 has been studied. The influence of SMM was compared with that of methionine. Addition of SMM to the medium at the concentration of 2.7 . 10(-5) M increased the incorporation of 5-methyl-3H-thymidine into DNA by about 20% and the incorporation of 2-14C-uridine into the total RNA of the cells by about 47%. The electrophoretic separation of RNA in polyacrylamide gels showed that SMM stimulated the incorporation of the label into 4 S, 5 S, 16 S and 23 S RNA with different intensity. The highest stimulation of the label incorporation has been observed in 16 S RNA.
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Reference: Lebenka A.Yu., Rachkus I.A., Kanopkaite S.I., Influence of S-methylmethionine on the metabolism of nucleic acids in Escherichia coli, Voprosy meditsinskoi khimii, 1981, vol: 27(2), 207-210.
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