Voprosy meditsinskoi khimii (ISSN 0042-8809)

Metabolic activation of N-methyl-N-nitrosourea in tumor cells

Abakumova O.Yu., Kutsenko N.G.
PubMed Id: 7281582
Year: 1981 vol: 27  issue:3  pages: 383-387
Abstract: An enzymatic activation of N-methyl-N-nitrose urea (MNU) was studied in microsomes, isolated from hepatoma 22a ascites cells, using a procedure which enabled to reveal the MNU enzymatic activation in mice liver microsomes. The MNU metabolic activation was not observed in hepatoma cell microsomes; at the same time, the MNU activation occurred when the cells were incubated under conditions of a suspension culture. The enzymes, participating in metabolic activation of MNU, were apparently arranged at the surface of the tumoral cells. Alkylation of MNU macromolecules was shown to be a two-step process. At the first step alkylation was due to spontaneous degradation of MNU and at the second step it was responsible for formation of active products as a result of MNU metabolic activation. MNU appears to induce synthesis of enzymes required of its metabolic activation in hepatoma 22a cells within 30 min.
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Reference: Abakumova O.Yu., Kutsenko N.G., Metabolic activation of N-methyl-N-nitrosourea in tumor cells, Voprosy meditsinskoi khimii, 1981, vol: 27(3), 383-387.