Voprosy meditsinskoi khimii (ISSN 0042-8809)

Simple, highly specific procedures for radioimmunochemical estimation of hypophyseal protein hormones

   
Isachenkov V.A., Badosov E.P., Krivosheev O.G., Nagornaia L.V., Karpenko E.K.
PubMed Id: 7281586
Year: 1981 vol: 27  issue:3  pages: 412-417
Abstract: Immunosorbents, prepared by means of polymerization of antiserum proteins using chlorcarbonic acid isobutyl ester, were used to increased reproducibility and simplicity of the radioimmunochemical procedures for estimation of the estimation of the hypophyseal hormones. In the types of the radioimmunoassay (RIA) studied the immunosorbents were prepared by means of polymerization of the first and second antibodies. The universal immunosorbent, suitable to estimation of various antigens, was prepared from the second antibodies. The procedure enabled to use as the first antibodies the blood sera with comparatively low antibody titre. High specificity of the RIA procedures described was demonstrated in estimation of bovine hypophyseal hormones - lactogenous (LTH), somatotropic (STH) and beta-lipotropine. The calibration curves were linear in the concentration range from 2 to 20 ng/ml for LTH, and from 1 to 10 ng/ml for STH and beta-lipotropine. Estimation of LTH by means of the RIA procedure required 3 hrs, and of STH and beta-lipotropine - 16 hrs.
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Reference: Isachenkov V.A., Badosov E.P., Krivosheev O.G., Nagornaia L.V., Karpenko E.K., Simple, highly specific procedures for radioimmunochemical estimation of hypophyseal protein hormones, Voprosy meditsinskoi khimii, 1981, vol: 27(3), 412-417.
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