Voprosy meditsinskoi khimii (ISSN 0042-8809)

Isolation and purification of urokinase

   
Kol'tsova S.V., Shataeva L.K., Sukhareva T.F., Byniaeva N.A., Fedorova Z.D.
PubMed Id: 7032062
Year: 1981 vol: 27  issue:5  pages: 623-626
Abstract: An improved procedure is developed for isolation of urokinase from human urine by means of ion exchange on cyrboxyl cationite SMT at pH 4.7. with subsequent stepwice elution by phosphate buffer, rH7.0. Depigmentation of the urine was achieved by pretreatment with an anionite FAF. Chromatographically pure preparation of urokinase was obtained as shown by gel filtration through Sephadex G-100 and G-150.
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Reference: Kol'tsova S.V., Shataeva L.K., Sukhareva T.F., Byniaeva N.A., Fedorova Z.D., Isolation and purification of urokinase, Voprosy meditsinskoi khimii, 1981, vol: 27(5), 623-626.
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