Voprosy meditsinskoi khimii (ISSN 0042-8809)

Method of quantitative determination of androgens and their metabolites

   
Degtiar' V.G., Miloserdov Iu.V., Kushlinskii N.E.
PubMed Id: 7157716
Year: 1982 vol: 28  issue:6  pages: 131-134
Abstract: Simple and rapid procedure is developed for quantitative estimation of 3H-androgen metabolites, which is carried out without extraction of labelled steroids from tissue homogenates after incubation with a substrate. After incubation the samples were liophylized and the labelled metabolites were separated using thin-layer chromatography on Silufol plates. The zones, corresponding to labelled metabolites, were scratched out with silica gelsheet and radioactivity was estimated. The procedure excluded practically a loss of labelled steroids in the samples. The procedure was used for estimation of 3H-androgen metabolites in vitro--3H-testosterone, 3H1 dihydrotestosterone and 3H-5 alpha-androsterone-3 beta, 17 beta-diol in homogenates of hypophyses and hypothalamuses from rat males (intact and castrated) and in adenoma of human prostatic gland.
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Reference: Degtiar' V.G., Miloserdov Iu.V., Kushlinskii N.E., Method of quantitative determination of androgens and their metabolites, Voprosy meditsinskoi khimii, 1982, vol: 28(6), 131-134.
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