Voprosy meditsinskoi khimii (ISSN 0042-8809)

Effect of slow rates of freezing on the functional state of mitochondria from various types of tissue preparations of kidney cortex

   
Subbota N.P., Belous A.M.
PubMed Id: 6836958
Year: 1983 vol: 29  issue:1  pages: 45-49
Abstract: Changes in functional activity of mitochondria, in concentration of respiration enzymes and the state of internal membrane after slow freezing down to -5 degrees, -12 degrees, -21 degrees and -30 degrees of various tissue preparations of renal cortex (homogenates, slices, etc) were studied. The following phenomena were found: an increase in the rate of mitochondrial respiration at the second metabolic state, inhibition of electron transport most distinct during mitochondrial oxidation of alpha-ketoglutarate, a decrease in concentration of cytochromes, an increase in the internal membrane permeability. Considerable damage of mitochondria was observed at temperatures between -5 degrees and -12 degrees but the most pronounced impairment of the organelles occurred at -10 degrees -20 degrees, i. e. at the freezing point of free water in the suspension and at eutectic temperatures.
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Reference: Subbota N.P., Belous A.M., Effect of slow rates of freezing on the functional state of mitochondria from various types of tissue preparations of kidney cortex, Voprosy meditsinskoi khimii, 1983, vol: 29(1), 45-49.
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