Voprosy meditsinskoi khimii (ISSN 0042-8809)

Effect of nonionic detergents on the results of studies of dehydrogenase and cholinesterase activities under conditions of enzyme electrophoresis

   
Nadirova T.Ya., Slutskiĭ I.P.
PubMed Id: 6858035
Year: 1983 vol: 29  issue:2  pages: 48-51
Abstract: Nonionic detergents of polyhydroxyethylene series were shown to catalyze the reduction of trinitrotetrazolium blue in reactions involving phenazinemethosulfate and other reducing agents. An approach of dehydrogenase fractions to the micells of nonionic detergents during enzyme gel electrophoresis led to an increase in colour intensity of individual isoenzymes and to redistribution of the isoenzyme spectrum as a result of catalytic effect of the detergents.
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Reference: Nadirova T.Ya., Slutskiĭ I.P., Effect of nonionic detergents on the results of studies of dehydrogenase and cholinesterase activities under conditions of enzyme electrophoresis, Voprosy meditsinskoi khimii, 1983, vol: 29(2), 48-51.
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