Voprosy meditsinskoi khimii (ISSN 0042-8809)

Radioimmune determination of steroids--comparison of 2 variants of the methods

   
Chaĭkovskiĭ V.S., Morozov V.I.
PubMed Id: 6880114
Year: 1983 vol: 29  issue:3  pages: 125-127
Abstract: Various conditions of radioimmunoassay (RIA) technique for estimation of 19-nortestosterone and methandrostenolone were studied. Dilution of antiserum, required for 50% binding of the labelled steroids, depended primarily on the RIA procedure used. When 19-nortestosterone was estimated by means of these two RIA procedures, the final dilution of antiserum was equal to 1:24,000 and 1:475,000, respectively. Characteristics of standard curves, sensitivity and accuracy were similar in these RIA procedures, but the rate of cross-reactions between antiserum and the steroids was different. Both RIA procedures indicated similar values of methandroctenolone and 19-nortestosterone content excreted with urine after oral administration of the steroids.
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Reference: Chaĭkovskiĭ V.S., Morozov V.I., Radioimmune determination of steroids--comparison of 2 variants of the methods, Voprosy meditsinskoi khimii, 1983, vol: 29(3), 125-127.
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