Voprosy meditsinskoi khimii (ISSN 0042-8809)

Spectrofluorimetric procedures for estimation of lysosomal cathepsins A, B C and D activities

   
Vasil'ev A.V., Kapelevich T.A., Tutel'ian V.A.
PubMed Id: 6880115
Year: 1983 vol: 29  issue:3  pages: 127-130
Abstract: Unified modifications of procedures are described for estimation of lysosomal cathepsins A, B, C and D activities. The procedures are based on spectrofluorimetric determination of beta-naphthylamine in the experiments with cathepsins B and C or on spectrofluorimetric determination of fluorescamine conjugates with epsilon-NH2-groups of free amino acids in the case of cathepsins A and D. Kinetics of all the reactions studied obeyed the Michaelis-Menten equation; the enzymatic activities could be estimated at protein concentration up to 0.1 mg/ml of the experimental sample.
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Reference: Vasil'ev A.V., Kapelevich T.A., Tutel'ian V.A., Spectrofluorimetric procedures for estimation of lysosomal cathepsins A, B C and D activities, Voprosy meditsinskoi khimii, 1983, vol: 29(3), 127-130.
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