Voprosy meditsinskoi khimii (ISSN 0042-8809)

Purification of commercial preparations of NADP from AMP

   
Nikulin I.R., Beliaeva N.F., Kagan Z.S.
PubMed Id: 6623988
Year: 1983 vol: 29  issue:4  pages: 128-131
Abstract: A method in described for elimination of AMP from commercially available preparations of NADP. The method enables to obtain desalted NADP preparations of 98-99.5% purity with a yield of 70-80% using only one chromatographic step. The procedure included anion exchange chromatography on Dowex 1, HCOO- -form. This chromatographic step completely separated AMP from NADP in a gradient of HCOOH concentrations from 0 to 2 N, and NADP was eluted by 1.5 N HCOOH containing 0.15 N HCOOK; during subsequent precipitation and washing of NADP by means of acetone HCOOK remained in a solution. Addition of HCOOK was required for a decrease in NADP elution volume which was important for the subsequent precipitation with acetone.
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Reference: Nikulin I.R., Beliaeva N.F., Kagan Z.S., Purification of commercial preparations of NADP from AMP, Voprosy meditsinskoi khimii, 1983, vol: 29(4), 128-131.
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