Voprosy meditsinskoi khimii (ISSN 0042-8809)

Improved procedure for isolation and purification of methionine gamma-lyase from Pseudomonas putida

   
Berezov T.T., Zanin V.A., Soda K.K., Esaki N.N., Shushe K.K.
PubMed Id: 6623989
Year: 1983 vol: 29  issue:4  pages: 131-135
Abstract: An improved, simplified and relatively rapid procedure is developed for isolation and purification of a new antitumor enzyme--methionine gamma-lyase from Pseudomonas putida. The method includes five steps instead of seven steps in previous procedure with a good yield of the enzyme. The purified enzyme was shown to be homogeneous by the criteria of disc gel electrophoresis. The highly homogeneous preparations of the enzyme exhibited the absorption maxima at 280 and 420 nm. The detailed studies on antileukemic activity of the methionine gamma-lyase are currently in progress.
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Reference: Berezov T.T., Zanin V.A., Soda K.K., Esaki N.N., Shushe K.K., Improved procedure for isolation and purification of methionine gamma-lyase from Pseudomonas putida, Voprosy meditsinskoi khimii, 1983, vol: 29(4), 131-135.
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