Voprosy meditsinskoi khimii (ISSN 0042-8809)

Isolation of triglyceridase from the aorta

Iusupova G.I.
PubMed Id: 6649537
Year: 1983 vol: 29  issue:5  pages: 93-96
Abstract: Isolation of triglyceridase from pig aorta was carried out by means of a following procedure: preparation of acetone powder, extraction of the enzyme in presence of sodium deoxycholate, ammonium sulfate fractionation, chromatography on Sepharose 6B, DEAE cellulose and cholate-Sepharose. The 1,000-fold purification of the enzyme was achieved. The purified preparation of triglyceridase was free of cholesterol esterase and proteinase activities.
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Reference: Iusupova G.I., Isolation of triglyceridase from the aorta, Voprosy meditsinskoi khimii, 1983, vol: 29(5), 93-96.