Voprosy meditsinskoi khimii (ISSN 0042-8809)

Isolation and quantitative analysis of ATP, synthesized by preparations of enriched plasma membrane portions from various tissues in the presence of insulin and somatotropin

   
Karelin A.A., Marchuk A.I., Rytvinskiĭ S.S.
PubMed Id: 6367210
Year: 1983 vol: 29  issue:6  pages: 99-105
Abstract: A transiently existent ATP, synthesized by the preparation enriched with plasmatic membranes of particles from rat skeletal muscles in presence of insulin 4 micrograms/ml and somatotropin 10-22 micrograms/ml within 1 min after addition of the hormones at 30 degrees, was isolated by means of chromatography on Dowex 1X8. The ATP was synthesized in a medium containing Tris-HCl buffer, pH 7.5, ADP, Mg2+ and P1 during NADH-dependent oxidation in presence of cytochrome c and oxygen; amount of the ATP was 0.1-0.3 nmole/mg/min. This form of ATP was isolated from particles enriched with plasmatic membranes and from plasmatic membranes of rat adipocytes, lymphocytes and liver tissue as well as of human erythrocytes within 1 min after addition of insulin into the mixture. The highest insulin-stimulated ATP-producing activity was found in plasmatic membranes of rat adipocytes--7.45 nmole/mg/min. Radiolabelled ATP, in presence of insulin transiently synthesized from ADP and 32P, was isolated from the preparation of human erythrocyte plasmatic membranes. Conditions of insulin-stimulated formation of the transient ATP in the preparation of human erythrocyte plasmatic membranes were studied.
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Reference: Karelin A.A., Marchuk A.I., Rytvinskiĭ S.S., Isolation and quantitative analysis of ATP, synthesized by preparations of enriched plasma membrane portions from various tissues in the presence of insulin and somatotropin, Voprosy meditsinskoi khimii, 1983, vol: 29(6), 99-105.
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