Voprosy meditsinskoi khimii (ISSN 0042-8809)

Fluorescent method of determining serotonin deaminating monoamine oxidase activity based on the amount of 5-hydroxyindoleacetic acid

   
Sominskiĭ V.N., Bluma R.K., Freĭmane T.Kh.
PubMed Id: 6204452
Year: 1984 vol: 30  issue:2  pages: 137-139
Abstract: A fluorimetric procedure is developed for estimation of serotonin deaminating activity of MAO by measuring the oxidized reaction product-5-hydroxyindolyl acetic acid (5-HIAA) extracted from acid solution by means of diethyl ester with subsequent transfer in 0.1 M phosphate buffer, pH 7.3. The optimal conditions for the extraction were developed, ensuring the maximal yield of 5-HIAA and serotonin. Low content of serotonin was found in the phosphate buffer. The concentration of serotonin was similar both in test and control samples; it did not interfere with the estimation of 5-HIAA. Sensitivity and reproducibility of 5-HIAA estimation by means of its fluorescence in 3 N HCI was higher as compared with the reaction with o-phthalic dialdehyde. Yield of 5-HIAA extracted from solution was approximately 90%. Activity of MAO, as shown by reaction of 5-HIAA with o-phthalic dialdehyde, was equal to 2.81 +/- 0.19 nM/mg/min (n = 15) and - by fluorescence in 3 N HCL - 2.92 +/- 0.29 nM/mg/min (n = 12).
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Reference: Sominskiĭ V.N., Bluma R.K., Freĭmane T.Kh., Fluorescent method of determining serotonin deaminating monoamine oxidase activity based on the amount of 5-hydroxyindoleacetic acid, Voprosy meditsinskoi khimii, 1984, vol: 30(2), 137-139.
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