Voprosy meditsinskoi khimii (ISSN 0042-8809)

Isolation and quantitative determination of apoprotein C-11 in the blood using rocket immunoelectrophoresis

   
Shcherbakova I.A., Petrova N.V., Metel'skaia V.A., Nikitina N.A., Chernysheva N.P.
PubMed Id: 6528541
Year: 1984 vol: 30  issue:6  pages: 82-88
Abstract: Apoprotein C-II was isolated from human blood plasma by means of column chromatography-gel filtration and DEAE ion exchange chromatography by modified method of Brown et al (1969). Production of antiserum to apoprotein C-II as well as a procedure for rocket immunoelectrophoresis are described for estimation of the apoprotein in blood plasma of men and women of various age, exhibiting different lipid spectra. Content of the apoprotein C-II in blood plasma, estimated by the immunoelectrophoretic procedure, was higher in men as compared with women and correlated with concentration of triglycerides in blood plasma.
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Reference: Shcherbakova I.A., Petrova N.V., Metel'skaia V.A., Nikitina N.A., Chernysheva N.P., Isolation and quantitative determination of apoprotein C-11 in the blood using rocket immunoelectrophoresis, Voprosy meditsinskoi khimii, 1984, vol: 30(6), 82-88.
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