Voprosy meditsinskoi khimii (ISSN 0042-8809)

A simple radioimmunological method of determining thymidine in biological fluids

   
Podgorodnichenko V.K., Konov A.V., Bryksina L.E., Mazurik V.K.
PubMed Id: 4049781
Year: 1985 vol: 31  issue:4  pages: 124-128
Abstract: A simple, sensitive and reproducible radioimmunoassay for determination of thymidine in biological fluids was developed. The technique is based on competition between 3H-thymidine and unlabelled thymidine for antithymidine antibodies. The cross-reactivity of structurally related compounds with the antibody was mostly negligible, except for deoxyuridine. The sensitivity of the assay was 10(-7) M. This radioimmunoassay may be used for measuring content of thymidine in blood serum and urine. A method for direct determination of thymidine in urine was not described before.
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Reference: Podgorodnichenko V.K., Konov A.V., Bryksina L.E., Mazurik V.K., A simple radioimmunological method of determining thymidine in biological fluids, Voprosy meditsinskoi khimii, 1985, vol: 31(4), 124-128.
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