Voprosy meditsinskoi khimii (ISSN 0042-8809)

Isolation and properties of chymotrypsin and trypsin labeled with iodine radioisotopes

   
Kulakov V.N., Korshunov V.B., Trifonenkova N.K., Bazhenova T.L., Sorokin V.P., Sedov V.V.
PubMed Id: 4090372
Year: 1985 vol: 31  issue:5  pages: 98-100
Abstract: Comparative study of iodination of chymotrypsin and trypsin was carried out using either iodine monochloride or the electrochemical procedure under conditions of controlled anode potential. It was shown that a part of iodine monochloride was consumed for oxidation of protein molecules. As a result of this process specific activity of the enzymes was decreased down to 50-60% of the initial activity after introduction of 2 atoms of iodine per a molecule. The similar degree of iodination under mild conditions of electrolysis in potentiostatic experiments caused considerably lower decrease in the enzymatic activity, reaching 5-10% of the initial activity (after introduction of I atom of iodine per a molecule--2.5%); labelling of trypsin and chymotrypsin by means of iodine radionuclides is promising for various medico-biological purposes.
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Reference: Kulakov V.N., Korshunov V.B., Trifonenkova N.K., Bazhenova T.L., Sorokin V.P., Sedov V.V., Isolation and properties of chymotrypsin and trypsin labeled with iodine radioisotopes, Voprosy meditsinskoi khimii, 1985, vol: 31(5), 98-100.
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