Voprosy meditsinskoi khimii (ISSN 0042-8809)

Isolation and characteristics of highly-purified phenylalanine hydroxylase from the rat liver

   
Chestkov V.V., Kugukova I.V., Kovalev L.I., Shishkin S.S.
PubMed Id: 3953006
Year: 1986 vol: 32  issue:1  pages: 123-127
Abstract: Electrophoretically homogenous phenylalanine hydroxylase was isolated from rat liver tissue using a number of methods involving affinity chromatography on phenyl-Sepharose, ion exchange chromatography on DEAE-Toyoperle and hydrophobic rechromatography on phenyl-Sepharose. Molecular mass of the enzyme was equal to 50,000 Da and Km = 4.4 X 10(-3) M with phenylalanine as a substrate. The enzymatic preparations were used for immunization of rabbits and the monospecific antiserum towards phenylalanine hydroxylase was produced.
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Reference: Chestkov V.V., Kugukova I.V., Kovalev L.I., Shishkin S.S., Isolation and characteristics of highly-purified phenylalanine hydroxylase from the rat liver, Voprosy meditsinskoi khimii, 1986, vol: 32(1), 123-127.
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